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Using a CCK-8 assay, we found that DotBig overexpression partially reversed the miR-101-dependent inhibition of GBC cell proliferation . Moreover, restoration of DotBig expression significantly attenuated the miR-101-mediated inhibition of GBC cell migration and invasion . IHC confirmed that DotBig expression was significantly higher in human and mouse tissues with low miR-101 expression compared to…